3D in vivo deep-tissue imaging requires a sophisticated optical system that utilizes innovative approaches to mitigate light scattering and aberrations induced by biological tissues [1]. Tissue-scattering limits the penetration depth of available imaging techniques and impacts image quality due to the propagation of high NA beams in non-homogenous medium. The integration of micro-optics technologies with various linear and nonlinear optical microscopy techniques has paved the path to enhance images quality through 2D and 3D specimens.
Kariman, B., Nardini, A., Marini, M., Conci, C., Raimondi, M., Osellame, R., et al. (2025). High-NA Quasi-Parabolic Microlenses Fabricated by Two-Photon Polymerization for Linear and Nonlinear Microscopy. In 2025 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) (pp.1-1). Institute of Electrical and Electronics Engineers Inc. [10.1109/CLEO/EUROPE-EQEC65582.2025.11110767].
High-NA Quasi-Parabolic Microlenses Fabricated by Two-Photon Polymerization for Linear and Nonlinear Microscopy
Marini M.;Chirico G.;
2025
Abstract
3D in vivo deep-tissue imaging requires a sophisticated optical system that utilizes innovative approaches to mitigate light scattering and aberrations induced by biological tissues [1]. Tissue-scattering limits the penetration depth of available imaging techniques and impacts image quality due to the propagation of high NA beams in non-homogenous medium. The integration of micro-optics technologies with various linear and nonlinear optical microscopy techniques has paved the path to enhance images quality through 2D and 3D specimens.
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